The 2004 LAM Conference

The 2004 LAM Conference
Cincinnati, Ohio
The Westin, downtown Cincinnati
March 26-28, 2004

Presented by: Rachel Squillace, Ph.D., Research Scientist

Immortalization of Human Tsc2 null AML Cells

Rachel Squillace¹*, Jane Yu², Sara Takacs¹, Rosa Rosado¹, John Wheeler¹, Suzanne Sobel¹, Bonnie E. Gould Rothberg¹,
Jonathan Rothberg¹, Elizabeth Henske², and Michael P. Weiner¹
¹The Rothberg Institute for Childhood Diseases, Guilford, CT 06437 and ²Fox Chase Cancer Center, Philadelphia, PA 19151

We have successfully generated several human angiomyolypoma (AML) cell lines and are in the process of generating lymphangioleiomyomatosis (LAM) lines to provide an in vitro cellular model for LAM and Tuberous Sclerosis Complex (TSC). Specifically, the goals of this project are to generate human LAM and AML cell lines along with a set of matching TSC gene knock-in control cell lines. We shall use these reagents for differential gene expression profiling, and to identify therapeutically beneficial compounds for LAM and TSC. We anticipate disseminating the generated cell lines to the research community. They represent a novel and important set of reagents that are needed to elucidate the molecular mechanisms of aberrant LAM and TSC cell behavior. The immortalized isogenic cell lines will find additional use in small molecule chemical screening and compound validation.

For further information contact: Rachel Squillace

Immortalized TSC2-null AML Cells

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